Expanded PersistenceT™ panels for deeper analysis of CAR T persistence

Adoptive cell therapy (ACT) with T-cells reprogrammed to express chimeric antigen receptors (CAR T-cells) bind specific antigens on the surface of cancer cells and trigger a cytotoxic response. CAR T-cell therapies have been successful in patients with hematological malignancies; however, patients remain at risk of relapse due to short-term persistence or non-expansion of CAR T-cells.1,2 This requirement for persistence is more evident in solid tumors as the hostile tumor microenvironment induces T-cell exhaustion.3,4 Studies have found that genomic and phenotypic features of CAR T-cells were major determinants of their persistence. Clinical results have shown that stemness and a memory-cell-like phenotype can promote sustained in vivo persistence of adoptively transferred CAR T-cells.3,4

Exploring orthotopic glioblastoma models to treat tumors and strengthen immune responses in the brain

Glioblastoma (GBM) patients are known to have a poor prognosis and tumor progression with high mortality and a median survival of only 12-15 months. Currently, anti-GBM therapy includes a multi-modality method involving radiotherapies and surgical removal of the tumor in combination with chemotherapy.1

Plate-based 3D culture assays that mimic the tumor microenvironment and predict in vivo responses

2D in vitro assays do not reflect the tumor architecture and environment found in vivo, resulting in differences in the outcomes and pharmacological potencies of test agents being screened when comparing 2D in vitro versus in vivo models.