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Cancer Type
Hematologic malignancies, Lymphoma
Technology Used
FISH
Turnaround Time
3-5 days
Use
Follicular lymphoma, Large B-cell lymphoma
Special Instructions
If aspiration syringe requires internal pre-coating to prevent clotting, diluted sodium heparin (green-top) tube should be utilized. Do not send syringes. 2-3 mL aseptically aspirated in sodium heparin (green-top) tube. Cautionary Note: Lithium heparin also comes in green-top collection tubes. Do not collect in lithium heparin.
Methodology
Fluorescence in situ hybridization (FISH)
Specimen Requirements
Information on collection, storage, and volume
Specimen
Bone marrow aspirate (preferred); whole blood (acceptable for limited tests); smear; slides; formalin-fixed paraffin embedded (FFPE) tissue block
Volume
3 mL bone marrow; 10 mL blood (adult), 5 mL blood (pediatric); fixed-cell pellet4-5 unstained slides cut to 4-5 microns thick, air-dried overnight; 0.2 cm x 0.2 cm x 0.2 cm tissue.
Container
Green-top (heparin) tube (optimal) or lavender-top (EDTA) tube
Storage Instructions
Refrigerate bone marrow and blood specimens; store slides and tissues at room temperature.
Causes for Rejection
Broken or dirty slides; excessive cellular debris or stained slides; frozen specimen; decalcified specimens. Do not collect in lithium heparin.
Collection
Refrigerate specimen. Use cold pack for transport. Be sure cold pack is not in direct contact with specimen during transport. All specimens should be shipped to Integrated Oncology on the day of collection. Delay in shipping may compromise cell viability and results. Air dry slides; Send in protective case to prevent damage. Tissue should be well-fixed in formalin. Do not decalcify. Store tissue specimens at room temperature (20-23.5° C). Use cold pack for transport. Be sure cold pack is not in direct contact with specimen during transport.
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