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Flow Cytometry: Hematolymphoid/Plasma Cell Neoplasia Assessment (HNA)(Immunophenotyping by Flow): Virtual (technical only)

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Synonyms Chronic Leukemia/Lymphoma Flow Cytometry Flow Immunophenotyping Leukemia Leukemia Profile Lymphoma

Test Details

Cancer Type

Acute myelocytic leukemia (AML), Chronic lymphocytic leukemia (CLL), Hematologic malignancies, Leukemia, Lymphoma, Myelodysplastic syndrome (MDS), Non-Hodgkin lymphoma (NHL)

Technology Used

FLOW

Turnaround Time

1-2 days
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Use

Characterization of hematopoietic cell populations, including leukemias and lymphomas using different cell surface and cytoplasmic antibodies. The technique allows for the lineage assignment of cells, maturational assessment of populations, detection of clonality, and detection of abnormal antigen expression of hematopoietic cells for diagnosis and monitoring of leukemia and lymphoma.Includes technical component only. For additional antibodies, refer to our Antibody Library.

Methodology

Immunophenotyping by flow cytometry

Specimen Requirements

Information on collection, storage, and volume

Specimen

Whole blood; bone marrow aspirate; tissue

Volume

3 mL whole blood; 2 mL bone marrow aspirate; 0.5-1.0 cm3 fresh tissue.

Container

Green-top (sodium heparin) tube (preferred), lavender-top (EDTA) tube, or yellow-top (ACD) tube for whole blood or bone marrow (acceptable, not preferred); fresh tissue in lymph node transport bottle containing RPMI

Storage Instructions

Room temperature

Causes for Rejection

Hemolysis; specimen clotted; specimen frozen; specimen in formalin or other fixative; blood more than 72 hours old; bone marrow aspirates more than five days old; bags or bottles of body fluid or bronchial washing; tissue in formalin or other fixative; contaminated transport medium

Collection

Store specimen at room temperature. DO NOT FREEZE.
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