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Aggressive B-Cell lymphoma FISH Profile

CPT Contact CPT coding department at 800-222-7566, ext. 6-8400.

Test Details

Cancer Type

Hematologic malignancies, Lymphoma, Non-Hodgkin lymphoma (NHL)

Technology Used

FISH

Turnaround Time

3 - 5 days

View Requisition Forms

Use

This test is a diagnostic test for non-Hodgkin's lymphoma (NHL) that detects primary genetic changes associated with various types of NHL, including high-grade B-cell lymphoma, follicular lymphoma (FL), Burkitt lymphoma (BL), diffuse large B-cell lymphoma (DLBCL) and those with BCL6 rearrangement.

Special Instructions

Indicate the pertinent clinical diagnosis and previous cytogenetic studies on the test request form. Note: This test number is used for tracking FISH specimens to the laboratory.

Test Includes

This test includes fluorescence in situ hybridization (FISH) for high-grade B-cell lymphoma, targeting rearrangement of BCL2, BCL6 and MYC.

Limitations

Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected. 

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Methodology

Fluorescence in situ hybridization (FISH)

Specimen Requirements

Information on collection, storage, and volume

Specimen

Whole blood, bone marrow, CSF, lymph node, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei, bone marrow touch preparation slides, paraffin slides or block are acceptable for testing.

Volume

5 mL whole blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 6 slides with metaphase and/or interphase nuclei or 6 touch preparation slides or 6 slides 4- or 5-micron thick with tumor

Minimum Volume

1 mL whole blood (pediatric), 1 mL bone marrow, fixed-cell pellet  from cytogenetic analysis, 3 slides with metaphase and/or interphase nuclei or 3 touch preparation slides or 3 slides 4- or 5-micron thick with tumor (Note: Minimum volumes may not allow for repeat testing.)

Container

Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube  (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity.

Storage Instructions

Transport to the testing laboratory at room temperature, use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze.

Causes for Rejection

Broken Vacutainer®; broken or stained slides; decalcified bone core; frozen specimen; quantity not sufficient for analysis

Collection

Utilize sterile technique and Labcorp transport kits when possible.

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