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FLT3 Mutation Analysis

CPT 81245; 81246
Synonyms
  • FLT3 D835 mutation
  • FLT3 Internal Tandem Duplication and Tyrosine Kinase Domain mutation
  • FLT3 ITD and D835 mutations
  • FLT3 ITD and TKD mutations
  • FLT3 ITD mutation
  • FLT3 TKD mutation

Test Details

Cancer Type

Acute myelocytic leukemia (AML), Hematologic malignancies

Technology Used

Molecular

Turnaround Time

3 - 5 days

View Requisition Forms

Use

Mutations in the FLT3 gene are common mutations in acute myeloid leukemia (AML). This assay detects internal tandem duplication (ITD) mutations and mutations in the tyrosine kinase domain (TKD) of FLT3. Presence of these mutations in AML provide prognostic information and can aid in the determination of therapeutic regimen.

Special Instructions

Please direct any questions regarding this test to customer service at 800-345-4363.

Limitations

This PCR assay is capable of detecting a mutant cell population with a sensitivity of 5 mutant cells per 100.

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Methodology

Polymerase chain reaction (PCR); restriction enzyme digest; capillary electrophoresis

Specimen Requirements

Information on collection, storage, and volume

Specimen

Whole blood, bone marrow or cell pellet

Volume

3-5 mL whole blood or 1-2 mL bone marrow

Minimum Volume

3 mL whole blood or 1 mL bone marrow

Container

Lavender-top (EDTA) tube, green-top (sodium heparin) tube, tan-top (K2-EDTA) tube or pink-top (K2-EDTA) tube

Storage Instructions

Refrigerate. If specimen is to be stored prior to shipment, store at 2°C to 8°C.

Causes for Rejection

Specimen does not meet collection criteria; frozen whole blood, marrow, or cell pellet; leaking tube; clotted blood or marrow; grossly hemolyzed specimen or otherwise visibly degraded; contamination by another specimen; specimens containing suspicious foreign material

Collection

Ship specimen at room temperature. Specimen should arrive in the laboratory within 48 hours of collection. Indicate date and time of collection on the request form.

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