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Cancer Type
Breast, Lung, Non-small cell lung cancer (NSCLC)
Technology Used
IHC
Turnaround Time
2 - 4 days
Use
The PD-L1 (SP142) is a qualitative IHC assay intended for use in the detection of PD-L1 in formalin-fixed, paraffin embedded (FFPE) in non-small-cell lung cancer and triple-negative breast cancer (TNBC) tissue samples using the Ventana BenchMark Ultra Platform.
Methodology
Immunohistochemistry (IHC)
Specimen Requirements
Information on collection, storage, and volume
Specimen
Formalin-fixed, paraffin-embedded tissue block or slides
Volume
One formalin-fixed, paraffin-embedded (FFPE) tissue block or four unstained positively charged slides sectioned from FFPE tissue block at 4 to 5 microns and be less than 2 months after sectioning if stored at 15-30°C.
Storage Instructions
Room temperature
Causes for Rejection
Insufficient tissue; inappropriate tissue source; incorrect specimen type; cytology samples are not accepted.
Collection
Recommended handling and processing conditions are: Recommended fixative is 10% neutral buffered formalin for a fixative time of at least 6 hours. Fixative times less than 6 hours results in a significant loss of staining intensity. Other fixatives are not recommended.
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