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BRAF V600E by IHC

CPT 88342, 88341

Test Details

Cancer Type

Colorectal cancer (CRC), Hairy cell leukemia (HCL), Melanoma, Thyroid

Technology Used

IHC

Turnaround Time

2-4 days

Use

This stain is used as an aid in the diagnosis of melanoma, hairy cell leukemia, and colorectal and thyroid carcinomas. 

Special Instructions

Clearly indicate clinical reason for testing provide the following information: differential diagnosis, site of specimen, and clinical information.

Test Includes

Option I: Slides and controls are returned for client interpretation
Option II: Slides and controls are returned with an immunostain interpretation
Option III: Full surgical pathology consultation (can be ordered with option II.)

Limitations

Use of fixatives other than 10% formalin may not yield equivalent or satisfactory results.

Methodology

Immunohistochemistry (IHC)

Specimen Requirements

Information on collection, storage, and volume

Specimen

Formalin-fixed paraffin embedded (FFPE) tissue block with Corresponding H&E, and/or unstained slides sectioned from tissue block. Precut slides from paraffin blocks in 5 micron sections.

Volume

0.2 cm x 0.2 cm x 0.2 cm tissue. Minimum of 2 slides per antibod in 5 micron sections

Storage Instructions

Store specimen at room temperature.

Collection

Tissue should be well-fixed in formalin. If alternative fixative is used, it should be noted on the requisition. Unstained Slides: Send all slides within 6 weeks of cutting.; Minimum of 2 slides per antibody. Mount on Poly-L-Lysine-coated or plus (+) slides. Air dry. Do not oven dry.