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Cancer Type
Colorectal cancer (CRC), Hairy cell leukemia (HCL), Melanoma, Thyroid
Technology Used
IHC
Turnaround Time
2-4 days
Use
This stain is used as an aid in the diagnosis of melanoma, hairy cell leukemia, and colorectal and thyroid carcinomas.
Special Instructions
Clearly indicate clinical reason for testing provide the following information: differential diagnosis, site of specimen, and clinical information.
Test Includes
Option I: Slides and controls are returned for client interpretation
Option II: Slides and controls are returned with an immunostain interpretation
Option III: Full surgical pathology consultation (can be ordered with option II.)
Limitations
Use of fixatives other than 10% formalin may not yield equivalent or satisfactory results.
Methodology
Immunohistochemistry (IHC)
Specimen Requirements
Information on collection, storage, and volume
Specimen
Formalin-fixed paraffin embedded (FFPE) tissue block with Corresponding H&E, and/or unstained slides sectioned from tissue block. Precut slides from paraffin blocks in 5 micron sections.
Volume
0.2 cm x 0.2 cm x 0.2 cm tissue. Minimum of 2 slides per antibod in 5 micron sections
Storage Instructions
Store specimen at room temperature.
Collection
Tissue should be well-fixed in formalin. If alternative fixative is used, it should be noted on the requisition. Unstained Slides: Send all slides within 6 weeks of cutting.; Minimum of 2 slides per antibody. Mount on Poly-L-Lysine-coated or plus (+) slides. Air dry. Do not oven dry.
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