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MET by FISH

CPT 88271, 88275, 88274 (use multipliers as necessary)

Test Details

Cancer Type

Lung, Non-small cell lung cancer

Technology Used

FISH

Turnaround Time

5-7 days
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Use

NSCLC

Special Instructions

Clearly indicate clinical reason for testing provide the following information: differential diagnosis, site of specimen, and clinical information.

Methodology

Fluorescence in situ hybridization (FISH)

Specimen Requirements

Information on collection, storage, and volume

Specimen

Slides; formalin-fixed paraffin embedded (FFPE) tissue block, or unstained slides sectioned from tissue block.  Precut slides from paraffin blocks in 4-5 micron sections.

Volume

3-4 unstained slides cut to 4 microns thick, air-dried overnight; 0.2 cm x 0.2 cm x 0.2 cm tissue.

Storage Instructions

Store slides and tissues at room temperature.

Causes for Rejection

Broken or dirty slides; excessive cellular debris or stained slides; frozen specimen; decalcified specimens

Collection

Tissue should be fixed in 10.0% neutral buffered formalin. The optimal fixation time for tissue is 6-48 hours. Do not decalcify. Store tissue specimens at room temperature (20-23.5° C). Use cold pack for transport. Be sure cold pack is not in direct contact with specimen during transport.
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