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CLL FISH Profile

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Synonyms
  • Chronic Lymphocytic Leukemia

Test Details

Cancer Type

Chronic lymphocytic leukemia (CLL), Hematologic malignancies, Leukemia

Technology Used

FISH

Turnaround Time

3 - 5 days

View Requisition Forms

Use

This test is a diagnostic test for chronic lymphocytic leukemia. Detection rate is improved from 45% with a chromosome study to 80% with fluorescence in situ hybridization (FISH). Differentiates CLL from MCL.

Special Instructions

Indicate pertinent clinical diagnosis and previous cytogenetic studies on the request form.

Test Includes

Fluorescence in situ hybridization (FISH) for chronic lymphocytic leukemia (CLL) and leukemic phase of mantle cell lymphoma (ATM, 12, 13q, 17p, and CCND1/IgH fusion)

Limitations

Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected. 

This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.

Methodology

Fluorescence in situ hybridization (FISH)

Specimen Requirements

Information on collection, storage, and volume

Specimen

Whole blood, bone marrow, CSF, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei or bone marrow touch preparation slides are acceptable for testing.

Volume

5 mL whole blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 6 slides with metaphase and/or interphase nuclei or 6 touch preparation slides

Minimum Volume

1 mL whole blood (pediatric), 1 mL bone marrow, fixed-cell pellet  from cytogenetic analysis, 3 slides with metaphase and/or interphase nuclei or 3 touch preparation slides (Note: Minimum volumes may not allow for repeat testing.)

Container

Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube (suboptimal). For samples with shared  karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity.

Storage Instructions

Transport to the testing laboratory at room temperature; use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze.

Causes for Rejection

Broken Vacutainer®; broken or stained slides; decalcified bone core; frozen specimen; quantity not sufficient for analysis

Collection

Utilize sterile technique and Labcorp transport kits when possible.

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