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Cancer Type
Acute myelocytic leukemia (AML), Hematologic malignancies, Leukemia
Technology Used
FISH
Turnaround Time
3 - 5 days
Use
This test is a diagnostic and prognostic test for AML in interphase cells as an adjunct to conventional karyotyping to identify AML-related abnormalities and for interphase analysis when conventional cytogenetic study of metaphase chromosomes fails.
Special Instructions
Indicate pertinent clinical diagnosis and previous cytogenetic studies on the test request form.
Test Includes
This test includes FISH for acute myeloid leukemia (AML), targeting rearrangement of PML/RARA, CBFB, RUNX1/RUNX1T1, KMT2A and numerical changes of 5q, 7q, 8, 8q and TP53.
Limitations
Molecular mutations or chromosomal alterations not targeted by the probe included in this profile will not be detected.
This test was developed and its performance characteristics determined by Labcorp. It has not been cleared or approved by the Food and Drug Administration.
Methodology
Fluorescence in situ hybridization (FISH)
Specimen Requirements
Information on collection, storage, and volume
Specimen
Whole blood, bone marrow, CSF, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei or bone marrow touch preparation slides are acceptable for testing.
Volume
5 mL whole blood (pediatric), 3 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 12 slides with metaphase and/or interphase nuclei or 12 touch preparation slides
Minimum Volume
1 mL whole blood (pediatric), 1 mL bone marrow, fixed-cell pellet from cytogenetic analysis, 6 slides with metaphase and/or interphase nuclei or 6 touch preparation slides (Note: Minimum volumes may not allow for repeat testing.)
Container
Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube (suboptimal). For samples with shared karyotype (510999) and any FISH study in the same tube, EDTA is not recommended due to the growth inhibition in cell culture. Adjust tube size to sample volume to avoid heparin toxicity.
Storage Instructions
Transport to the testing laboratory at room temperature; use of a cool pack or a Labcorp transport kit is acceptable. Do not allow samples to overheat or freeze.
Causes for Rejection
Broken Vacutainer®; broken or stained slides; decalcified bone core; frozen specimen; quantity not sufficient for analysis
Collection
Utilize sterile technique and Labcorp transport kits when possible.
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