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ALL FISH Profile (Pediatric)

CPT Contact CPT coding department at 800-222-7566, ext 6-8400.
Synonyms
  • Acute Lymphocytic Leukemia (ALL), Pediatric

Test Details

Cancer Type

Acute lymphocytic leukemia (ALL), Hematologic malignancies, Leukemia

Technology Used

FISH

Turnaround Time

3 - 5 days

View Requisition Forms

Use

Diagnostic and prognostic test for acute lymphoblastic leukemia in the pediatric population; detection rate is improved from 50% with a chromosome study to 90% with FISH

Special Instructions

Indicate pertinent clinical diagnosis and previous cytogenetic studies on the test request form.

Test Includes

Fluorescence in situ hybridization (FISH) for acute lymphoblastic leukemia (ALL), targeting rearrangement of BCR-ABL, TCF3, KMT2A (MLL), ETV6/RUNX1, and numerical changes of 4, 10, 17, and CDKN2A deletion.

Limitations

Molecular mutations or chromosomal alterations not targeted by the probes included in this profile will not be detected.

Methodology

Fluorescence in situ hybridization (FISH)

Specimen Requirements

Information on collection, storage, and volume

Specimen

Blood, bone marrow, CSF, fixed-cell pellet from a cytogenetic analysis, slide with metaphase and/or interphase nuclei or bone marrow touch preparation slides are acceptable for testing.

Volume

5 mL blood (pediatric), 3 mL bone marrow, fiexed-cell pellet from a cytogenetic analysis, 12 slides with metaphase and/or interphase nuclei or 12 touch preparation slides.

Minimum Volume

1 mL blood (pediatric), 1 mL bone marrow, fixed-cell pellet from a cytogenetic analysis, 6 slides with metaphase and/or interphase nuclei or 6 touch preparation slides. (Note: Minimum volumes may not allow for repeat testing.)

Container

Green-top (sodium heparin) tube; pediatric Vacutainer® is optimal or lavender-top (EDTA) tube (suboptimal). Adjust tube size to sample volume to avoid heparin toxicity.

Storage Instructions

Maintain the specimen at either room temperature or refrigerate. Do not freeze.

Causes for Rejection

Broken Vacutainer®; broken or stained slides; paraffin blocks; paraffin slides; decalcified bone cores; frozen specimen; quantity not sufficient for analysis

Collection

Transport to the testing laboratory at room temperature, use of a cool pack or a LabCorp transport kit is acceptable. Do not allow samples to overheat or freeze.

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