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Cancer Type
Brain, Glioblastoma
Technology Used
Molecular
Turnaround Time
7 - 10 days
Use
MGMT (O6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that is involved in the repair of damage caused by a variety DNA cross-linking compounds, including alkylating agents. Increased methylation of the MGMT gene promoter region causes diminished or silenced expression of the gene, making cells more sensitive to DNA damage. The relationship has been shown for glioblastomas and alkylating agents such as temozolomide (Temodar®).
Special Instructions
Please provide a copy of the pathology report. Sample submission without a copy of the pathology report may delay testing. Please direct any questions regarding this test to customer service at 800-345-4363.
Limitations
Preparation of DNA from tissue samples is dependent upon the quality of the provided specimen. Inadequate DNA extraction may occur in a significant number of paraffin-embedded samples.
Methodology
Quantitative methylation-specific polymerase chain reaction (PCR)
Specimen Requirements
Information on collection, storage, and volume
Specimen
Glioblastoma specimens in formalin-fixed, paraffin-embedded (FFPE) tissue blocks or slides
Volume
FFPE tissue block or eight unstained slides at 5 μM and one matching H&E slide or nine precut unstained slides from a paraffin block in 5-μM sections
Minimum Volume
50mm2 total tumor area is required for testing. Two unstained slides at 5 μM and one matching H&E slide or three unstained slides at 5 μM.
Storage Instructions
Maintain blocks/slides at room temperature.
Causes for Rejection
Tissue is not primary brain tumor; tumor block containing insufficient tumor tissue; tumor fixed in a heavy metal fixative; broken or stained slides; decalcified specimens
Collection
FFPE tissue block or eight unstained slides at 5 μM and one matching H&E slide or nine precut unstained slides from a paraffin block in 5-μM sections
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