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MGMT Gene Methylation Assay

CPT 81287; 88381
Synonyms
  • O6-Methylguanine-DNA Methyltransferase (MGMT)

Test Details

Cancer Type

Brain, Glioblastoma

Technology Used

Molecular

Turnaround Time

7 - 10 days

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Use

MGMT (O6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that is involved in the repair of damage caused by a variety DNA cross-linking compounds, including alkylating agents. Increased methylation of the MGMT gene promoter region causes diminished or silenced expression of the gene, making cells more sensitive to DNA damage. The relationship has been shown for glioblastomas and alkylating agents such as temozolomide (Temodar®).

Special Instructions

Please provide a copy of the pathology report. Sample submission without a copy of the pathology report may delay testing. Please direct any questions regarding this test to customer service at 800-345-4363.

Limitations

Preparation of DNA from tissue samples is dependent upon the quality of the provided specimen. Inadequate DNA extraction may occur in a significant number of paraffin-embedded samples.

Methodology

Quantitative methylation-specific polymerase chain reaction (PCR)

Related Documents

For more information, please view the literature below.

MGMT Gene Methylation Assay: A Biomarker for Therapy Consideration in Glioblastoma

    Specimen Requirements

    Information on collection, storage, and volume

    Specimen

    Glioblastoma specimens in formalin-fixed, paraffin-embedded (FFPE) tissue blocks or slides

    Volume

    FFPE tissue block or eight unstained slides at 5 μM and one matching H&E slide or nine precut unstained slides from a paraffin block in 5-μM sections

    Minimum Volume

    50mm2 total tumor area is required for testing. Two unstained slides at 5 μM and one matching H&E slide or three unstained slides at 5 μM.

    Storage Instructions

    Maintain blocks/slides at room temperature.

    Causes for Rejection

    Tissue is not primary brain tumor; tumor block containing insufficient tumor tissue; tumor fixed in a heavy metal fixative; broken or stained slides; decalcified specimens

    Collection

    FFPE tissue block or eight unstained slides at 5 μM and one matching H&E slide or nine precut unstained slides from a paraffin block in 5-μM sections

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