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Cancer Type
Hematologic malignancies, Lymphoma
Technology Used
Molecular
Turnaround Time
5 - 7 days
Use
Diagnosis and monitoring of follicular lymphoma and diffuse large B-cell lymphoma
Limitations
The absence of a detectable fusion product does not exclude the presence of a rearrangement outside of the two breakpoint regions evaluated, or the presence of a B-cell lymphoma without an underlying BCL2-IGH translocation. In addition, it is important to note that the BCL2-IGH gene rearrangement has also been detected at low levels in some apparently benign hyperplastic lymphoid tissues.
Methodology
Molecular Polymerase chain reaction (PCR)
Specimen Requirements
Information on collection, storage, and volume
Specimen
Whole blood, bone marrow, fresh or frozen tissue, fixed-cell pellet
Volume
7 mL whole blood, 2 mL bone marrow, or 0.5g tissue
Container
Lavender-top (EDTA) tube, yellow-top (ACD-A) tube, or tissue in lymph node transport bottle
Storage Instructions
Maintain specimen at room temperature. Some specimen types may be submitted frozen; call client services for instructions.
Causes for Rejection
Specimen more than 48 hours old; clotted blood
Collection
Indicate date and time of collection on the request form. To avoid delays in turnaround time when requesting multiple tests on frozen samples, please submit separate frozen specimens for each test requested.
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