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Flow Cytometry:Hematolymphoid/Plasma Cell Neoplasia Assessment (HNA) (Immunophenotyping by Flow)

CPT For CPT coding, call 800-222-7566, ext 6-8400.
Synonyms
  • Comp panel: Leukemia/Lymphoma
  • Flow Cytometry
  • Flow Cytometry Panel
  • Flow Cytometry Report
  • Flow Immunophenotyping
  • Hematoymphoid Neoplasm Profile
  • Leukemia
  • Leukemia Panel
  • Leukemia Profile
  • Leukemia/Lymphoma Evaluation
  • Leukemia/Lymphoma Profile
  • Lymphoma
  • Lymphoma Panel
  • Lymphoma Profile
  • Plasma Cell Neoplasm Profile

Test Details

Cancer Type

Acute myelocytic leukemia (AML), Chronic lymphocytic leukemia (CLL), Hematologic malignancies, Leukemia, Lymphoma, Myelodysplastic syndrome (MDS), Non-Hodgkin lymphoma (NHL)

Technology Used

FLOW

Turnaround Time

1 - 3 days

View Requisition Forms

Use

Identify and characterize the following:

• Mature B-Cell lymphoproliferative disorders (chronic lymphocytic leukemia (CLL), mantle cell lymphoma, monoclonal B-cell lymphocytosis, hairy cell leukemia, B-cell lymphomas)

• Mature T-cell and natural killer cell lymphoproliferative disorders (T-cell lymphomas, Sezary syndrome/mycosis fungoides. T-cell and NK cell large granular lymphocyte disorders, adult T-cell leukemia/lymphoma)

• Acute leukemia (acute myeloid leukemia, precursor lymphoid leukemia, acute leukemia of ambiguous lineage)

• Plasma cell neoplasm (multiple myeloma, plasmacytoma, monoclonal Gammopathy)

Special Instructions

If both tissue flow cytometry and histology are required, submit one portion of fresh specimen in transport medium or saline for flow cytometry and one portion in 10% formalin for histologic analysis. A histology test number is required for tissue specimens.

Please direct any questions regarding this test to oncology customer service at 800-345-4363. Pathologist consultation is available Monday through Friday. Acute leukemia reporting is available on the weekend if requested. Please call Friday to coordinate. Indicate differential diagnosis on test request form. Submit recent CBC results for correlation. Billing will be performed back end.

Methodology

Immunophenotyping by flow cytometry

Related Documents

For more information, please view the literature below.

Leukemia & Lymphoma Management Test Selection Table

    Specimen Requirements

    Information on collection, storage, and volume

    Specimen

    Whole blood, bone marrow aspirate, body fluids, fresh lymph node, spleen, extranodal solid tissue, biopsy, or needle aspirate

    Volume

    3 mL whole blood or 2 mL bone marrow aspirate, 2 mL body fluid tube. Large volumes of body fluids should be concentrated to <5 mL; 0.5 to 1.0 cm3 fresh tissue.

    Minimum Volume

    1 mL whole blood or bone marrow (Note: This volume does not allow for repeat testing.)

    Container

    Green-top (sodium heparin) tube (preferred), lavender-top (EDTA) tube, or yellow-top (ACD) tube for whole blood or bone marrow (acceptable, not preferred); lavender-top (EDTA body fluids) tube; fresh tissue in lymph node transport bottle containing RPMI

    Storage Instructions

    Non-New York state specimens: Maintain specimen at room temperature. New York state specimens: Ship with a refrigerated (not frozen) gel pack in shipper kit.

    Causes for Rejection

    Hemolysis; specimen clotted; specimen frozen; specimen in formalin or other fixative; blood (non-New York state specimens) more than 72 hours old; blood (New York state specimens) more than 48 hours old; bone marrow aspirates more than five days old; bags or bottles of body fluid or bronchial washing; tissue in formalin or other fixative; contaminated transport medium

    Collection

    Note: In an attempt to maintain specimen at room temperature,

    • In hot weather, enclose a refrigerated (not frozen) gel pack in shipper kit.

    • In cold weather, run hot water over gel pack for three to five minutes and enclose in shipper kit.

    • Ship specimens in a timely manner based on the specific requirements of the test.

    Submit blood or bone marrow at room temperature. Collect the specimen so it will arrive in the laboratory Monday through Saturday and within 24 hours of collection. Please state on the test request form the date and time of collection and the name and phone number of the pathologist responsible for the histologic or cytologic diagnosis.

    For fresh tissue, aseptically cut tissue in pieces and place in lymph node transport bottle. If aspirate is submitted, rinse needle in transport medium. Submit at room temperature using Lymph Node Transport Kit (supplied by Labcorp). If transport kit is not available, place specimen in sterile container with saline. Submit specimen so it will arrive in the laboratory Monday through Saturday and within 24 hours of surgical removal. To avoid transportation delays, submit specimen on the day of collection.

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