Abstract: Comprehensive Genomic Profiling (CGP) has emerged as a progressive standard of care for the understandingclinical oncology and treatment of solid tumors (Conroy, Pabla et al. 2021). By identifying actionable mutationsthrough next-generation sequencing of solid tumors CGP enables targeted therapy decisions. Formalin-fixedparaffin-embedded tissues are notoriously difficult samples in respect to reliably extracting high-qualitynucleic acids and sufficient genetic material to meet sequencing input requirements. In this work we presentan automated solution for upstream sample processing of solid tumors to enable high throughput and scalableCGP workflows by eliminating bottlenecks and enhancing results. The Sonication STAR automated DNA and RNAmethods from FFPE Tissues was created as a collaboration between Hamilton Company, Covaris, and Labcorpand it offers the potential reduction in quantity not sufficient (QNS) samples and sequencing performance improvementsresulting in a 16 % increase in fully reported tumor profiles for patients. This automated approachoffers potential saving in workflow costs, improved efficiency, and a reduction in re-extraction and re-sequencingof tumors.

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Published in: SLAS Technology