Automation of PGDx elio® tissue complete on the Beckman Coulter Biomek NGeniuS System

The PGDx elio® tissue complete (ETC) assay is an FDA-approved, comprehensive next-generation sequencing (NGS) assay designed for tumor profiling. Available as a decentralized kit, it utilizes DNA extracted from formalin-fixed paraffin-embedded (FFPE) tumor tissue and requires approximately six hours of hands-on time, and three days of laboratory processing by qualified personnel. Next-generation sequencing (NGS) workflows often involve detail oriented multistep procedure that are susceptible to human error and can limit the sample throughput. Introducing automated liquid handling systems can improve efficiency, streamline complex procedure and enhance reproducibility. The Beckman Coulter Biomek NGeniuS is a compact benchtop liquid handling system developed for NGS library preparation. This instrument is a costeffective choice that offers a fixed deck layout and low dead volume requirements compared to other automated platforms on the market. The goal for this study to compare the performance between the standard PGDx ETC manual process and an automated workflow on the Biomek NGeniuS system.